MANF Protein
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- Target See all MANF Proteins
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Application
- Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)
- Characteristics
- Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke
- Purification
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Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy. - Endotoxin Level
- Less than 1EU/µg of protein as determined by LAL method
- Top Product
- Discover our top product MANF Protein
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Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (Active) protein
MANF Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 25-182) protein (His tag)MANF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) (AA 1-182) protein (GST tag)MANF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (His tag)MANF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) protein (Myc-DYKDDDDK Tag)MANF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
- Buffer
- PBS pH 7.4
- Handling Advice
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
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- Target
- MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))
- Alternative Name
- MANF (MANF Products)
- Background
- MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- Molecular Weight
- Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
- UniProt
- P55145
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