CDNF Protein
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- Target See all CDNF Proteins
- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- CHO Cells
- Application
- Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)
- Characteristics
- Biological properties: Protein is biologically active. Suitable for cell culture and in vivo testing. Biological activity of human CDNF was tested in rat 6-OHDA model of Parkinson’s disease and shown to be both neuroprotective and neurorestorative (for methods see: Lindholm et al. 2007, Voutilainen et al. 2011)
- Purification
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Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Protein is sterile-filtrated through 0.22 µg filter.
Purified human CDNF is characterized as homogenous material by mass-spectroscopy. - Endotoxin Level
- Less than 1EU/µg of protein as determined by LAL method
- Top Product
- Discover our top product CDNF Protein
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Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 25-187) protein (His tag)
CDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cerebral Dopamine Neurotrophic Factor (CDNF) protein (His tag)CDNF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Cerebral Dopamine Neurotrophic Factor (CDNF) proteinCDNF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Comment
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Synonyms: Cerebral dopamine neurotrophic factor - CDNF, ARMETL1.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
- Buffer
- PBS pH 7.5
- Handling Advice
- Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
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- Target
- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
- Alternative Name
- CDNF (CDNF Products)
- Background
- CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007, Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
- Molecular Weight
- Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
- UniProt
- Q49AH0
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