ENO1 Protein (AA 1-434) (GST tag)
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- Target See all ENO1 Proteins
- ENO1 (Enolase 1 (ENO1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-434
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This ENO1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- ENO1 (Human) Recombinant Protein (P01)
- Sequence
- MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEGLGSK AKFAGRNFRN PLAK
- Characteristics
- Human ENO1 full-length ORF ( AAH11130.1, 1 a.a. - 434 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens." in: Journal of proteomics, Vol. 74, Issue 10, pp. 2008-17, (2011) (PubMed).
: "Antifibroblast antibodies from systemic sclerosis patients bind to {alpha}-enolase and are associated with interstitial lung disease." in: Annals of the rheumatic diseases, Vol. 69, Issue 2, pp. 428-33, (2010) (PubMed).
: "Melanoma-associated retinopathy: a paraneoplastic autoimmune complication." in: Archives of ophthalmology, Vol. 127, Issue 12, pp. 1572-80, (2009) (PubMed).
: "Anti-alpha-enolase antibodies in patients with inflammatory Bowel disease." in: Clinical chemistry, Vol. 54, Issue 3, pp. 534-41, (2008) (PubMed).
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Direct characterization of target podocyte antigens and auto-antibodies in human membranous glomerulonephritis: Alfa-enolase and borderline antigens." in: Journal of proteomics, Vol. 74, Issue 10, pp. 2008-17, (2011) (PubMed).
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- Target
- ENO1 (Enolase 1 (ENO1))
- Alternative Name
- ENO1 (ENO1 Products)
- Synonyms
- enolase 1 Protein, F2P9.10 Protein, F2P9_10 Protein, Pgh1 Protein, pENO1 Protein, enolase 1 Protein, phosphopyruvate hydratase Protein, ENO1 Protein, eno1 Protein
- Background
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Full Gene Name: enolase 1, (alpha)
Synonyms: ENO1L1,MBP-1,MPB1,NNE,PPH - Gene ID
- 2023
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