CDK4 Protein (AA 1-303) (GST tag)
(1 reference)-
- Target See all CDK4 Proteins
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-303
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CDK4 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CDK4 (Human) Recombinant Protein (P02)
- Sequence
- MATSRYEPVA EIGVGAYGTV YKARDPHSGH FVALKSVRVP NGGGGGGGLP ISTVREVALL RRLEAFEHPN VVRLMDVCAT SRTDREIKVT LVFEHVDQDL RTYLDKAPPP GLPAETIKDL MRQFLRGLDF LHANCIVHRD LKPENILVTS GGTVKLADFG LARIYSYQMA LTPVVVTLWY RAPEVLLQST YATPVDMWSV GCIFAEMFRR KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE
- Characteristics
- Human CDK4 full-length ORF ( AAH03644, 1 a.a. - 303 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Cyclin-Dependent Kinase 4 (CDK4) (AA 207-303) protein (His-IF2DI Tag)
CDK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase 4 (CDK4) (AA 1-303) protein (His tag)CDK4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cyclin-Dependent Kinase 4 (CDK4) (AA 211-303) protein (GST tag)CDK4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 4 (CDK4) proteinCDK4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Cyclin-Dependent Kinase 4 (CDK4) protein (Myc-DYKDDDDK Tag)CDK4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors"." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 5070-85, (2006) (PubMed).
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: "Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors"." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 5070-85, (2006) (PubMed).
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- Target
- CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
- Alternative Name
- CDK4 (CDK4 Products)
- Synonyms
- zgc:153726 Protein, CMM3 Protein, PSK-J3 Protein, Crk3 Protein, 8-6 Protein, CDK4 Protein, CDK4/6 Protein, CG5072 Protein, Cdk4/6 Protein, DmCdk4 Protein, Dmel\\CG5072 Protein, Pk53C Protein, Pk?7 Protein, cdk Protein, cdk4 Protein, cdk4/6 Protein, dCdk4 Protein, l(2)05428 Protein, l(2)0671 Protein, l(2)k06503 Protein, l(2)s4639 Protein, l(2)sh0671 Protein, xcdk4 Protein, cyclin-dependent kinase 4 Protein, cyclin dependent kinase 4 Protein, Cyclin-dependent kinase 4 Protein, cyclin-dependent kinase 4 L homeolog Protein, cdk4 Protein, CDK4 Protein, Cdk4 Protein, cdk4.L Protein
- Background
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Full Gene Name: cyclin-dependent kinase 4
Synonyms: CMM3,MGC14458,PSK-J3 - Gene ID
- 1019
- Pathways
- Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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