BRAF Protein (AA 346-445) (GST tag)

Catalog No. ABIN1346843

Product Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Protein Type: Recombinant

Protein Characteristics: AA 346-445

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This BRAF protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: BRAF (Human) Recombinant Protein (Q01)

Sequence: FRPADEDHRN QFGQRDRSSS APNVHINTIE PVNIDDLIRD QGFRGDGGST TGLSATPPAS LPGSLTNVKA LQKSPGPQRE RKSSSSSEDR NRMKTLGRRD

Characteristics: Human BRAF partial ORF ( NP_004324, 346 a.a. - 445 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF (BRAF Products)

Synonyms: B-RAF1 Protein, BRAF1 Protein, NS7 Protein, RAFB1 Protein, C-RMIL Protein, RMIL Protein, B-Raf Protein, braf1 Protein, rafb1 Protein, b-raf1 Protein, sierrabraf Protein, BRAF Protein, 9930012E13Rik Protein, AA120551 Protein, AA387315 Protein, AA473386 Protein, B-raf Protein, Braf-2 Protein, Braf2 Protein, C230098H17 Protein, C87398 Protein, D6Ertd631e Protein, braf Protein, fc48g01 Protein, wu:fc48g01 Protein, B-Raf proto-oncogene, serine/threonine kinase Protein, B-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Braf transforming gene Protein, si:dkey-222f8.3 Protein, BRAF Protein, Braf Protein, braf.S Protein, braf Protein, si:dkey-222f8.3 Protein

Background: Full Gene Name: v-raf murine sarcoma viral oncogene homolog B1
Synonyms: B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1

Gene ID: 673

NCBI Accession: NM_004333

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy