LAG3 Protein (Fc Tag)
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- Target See all LAG3 Proteins
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human, Mouse, Monkey
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Source
- CHO Cells
- Purification tag / Conjugate
- This LAG3 protein is labelled with Fc Tag.
- Application
- SDS-PAGE (SDS), Flow Cytometry (FACS)
- Specificity
- Binds to human, mouse and monkey MHC class II.
- Cross-Reactivity
- Human, Monkey, Mouse (Murine)
- Characteristics
- The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1.
- Purity
- >99 % (SDS-PAGE)
- Endotoxin Level
- <0.1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product LAG3 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Flow Cytometry: Detection of MHC class II molecules in combination with fluorescently labeled antibodies to IgG1. Uni-Prot link P18627: LAG-3 (human) http://www.uniprot.org/uniprot/P18627 .Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 4202-11, (2007) (PubMed).
: "A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors." in: Cancer letters, Vol. 235, Issue 1, pp. 147-53, (2006) (PubMed).
: "LAG-3 (CD223) reduces macrophage and dendritic cell differentiation from monocyte precursors." in: Immunology, Vol. 114, Issue 3, pp. 369-74, (2005) (PubMed).
: "Maturation and activation of dendritic cells induced by lymphocyte activation gene-3 (CD223)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 8, pp. 3874-80, (2002) (PubMed).
: "T cell major histocompatibility complex class II molecules down-regulate CD4+ T cell clone responses following LAG-3 binding." in: European journal of immunology, Vol. 26, Issue 5, pp. 1180-6, (1996) (PubMed).
: "CD4/major histocompatibility complex class II interaction analyzed with CD4- and lymphocyte activation gene-3 (LAG-3)-Ig fusion proteins." in: European journal of immunology, Vol. 25, Issue 9, pp. 2718-21, (1995) (PubMed).
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A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 4202-11, (2007) (PubMed).
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- Target
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Alternative Name
- LAG-3 (LAG3 Products)
- Synonyms
- CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein
- Background
- Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
- Molecular Weight
- ~80kDa (SDS-PAGE)
- UniProt
- P18627
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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