Flagellin Protein (FliC)
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- Target See all Flagellin (FliC) Proteins
- Flagellin (FliC)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human, Mouse
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Source
- Salmonella typhimurium
- Application
- SDS-PAGE (SDS)
- Specificity
- Binds to human and mouse TLR5 (Toll-like receptor 5).
- Cross-Reactivity
- Human, Mouse (Murine)
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product FliC Protein
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Flagellin (FliC) protein
FliC Origin: Borrelia burgdorferi Host: Escherichia coli (E. coli) Recombinant ELISA, WB
Flagellin (FliC) (AA 2-498) protein (His tag)FliC Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 100 μL sterile water.
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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- Target
- Flagellin (FliC)
- Alternative Name
- Flagellin (FliC Products)
- Synonyms
- hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein
- Target Type
- Tag
- Background
- Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
- UniProt
- P06179
- Pathways
- Inflammasome
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