Netrin 1 Protein (NTN1) (AA 25-604) (DYKDDDDK Tag)
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- Target See all Netrin 1 (NTN1) Proteins
- Netrin 1 (NTN1)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-604
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Origin
- Mouse, Human, Rat
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Netrin 1 protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Specificity
- Binds to human, mouse and rat UNC5B.
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Characteristics
- Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 100 μL sterile distilled water.
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance." in: Nature communications, Vol. 6, pp. 7398, (2015) (PubMed).
: "
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Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance." in: Nature communications, Vol. 6, pp. 7398, (2015) (PubMed).
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- Target
- Netrin 1 (NTN1)
- Alternative Name
- Netrin-1 (NTN1 Products)
- Synonyms
- ntn1-A Protein, Netrin-1 Protein, NTN1 Protein, unc-6 Protein, MGC147073 Protein, NTN1L Protein, AI561871 Protein, BC019633 Protein, netrin-1 Protein, netrin 1 L homeolog Protein, netrin 1 Protein, ntn1.L Protein, NTN1 Protein, ntn1 Protein, Ntn1 Protein
- Background
- Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
- Molecular Weight
- ~80kDa (SDS-PAGE)
- UniProt
- O95631
- Pathways
- Regulation of Cell Size
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