LIF Protein (AA 23-202)

Catalog No. ABIN1344370

Product Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-202

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Specificity: Mouse and human embryonic stem cells.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: Human LIF (aa 23-202) is fused at the N-terminus to a tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.05EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF (LIF Products)

Synonyms: CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein

Background: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130 kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Molecular Weight: ~46kDa (SDS-PAGE)

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding