CD40 Ligand Protein (CD40LG) (AA 18-111) (DYKDDDDK Tag)
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- Target See all CD40 Ligand (CD40LG) Proteins
- CD40 Ligand (CD40LG)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 18-111
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Origin
- Human
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Source
- CHO Cells
- Purification tag / Conjugate
- This CD40 Ligand protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Brand
- MegaCD40L™
- Specificity
- Binds to human CD40.
- Cross-Reactivity
- Human
- Characteristics
- Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product CD40LG Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3768-77, (2015) (PubMed).
: "Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sézary syndrome: circumvention by hexameric soluble CD40L." in: Blood, Vol. 105, Issue 1, pp. 219-25, (2004) (PubMed).
: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).
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CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3768-77, (2015) (PubMed).
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- MegaCD40L (CD40LG Products)
- Synonyms
- CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
- Background
- MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
- Molecular Weight
- ~35-40kDa (SDS-PAGE)
- UniProt
- P29965
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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