IDO1 Protein (AA 1-407) (His tag)
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- Target See all IDO1 Proteins
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-407
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IDO1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Mouse (Murine)
- Characteristics
- Mouse IDO (aa 1-407) is fused at the C-terminus to a His-tag.
- Purity
- >90 % (SDS-PAGE)
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- <1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product IDO1 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Specific Activity: >100'000U/mg protein with L-tryptophan as substrate and after addition of 7μM hemin for protein induction. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.2μm-filtered solution in 50 mM TRIS-Cl, pH 7.4, containing 1 mM EDTA.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C. - Expiry Date
- 3 months
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- Target
- IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))
- Alternative Name
- IDO (IDO1 Products)
- Synonyms
- INDO Protein, ido Protein, indo Protein, IDO Protein, cd107b Protein, IDO1 Protein, IDO-1 Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, indoleamine 2,3-dioxygenase 1 L homeolog Protein, indoleamine 2,3-dioxygenase 1-like Protein, IDO1 Protein, ido1.L Protein, ido1 Protein, Ido1 Protein, LOC100050681 Protein
- Background
- IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
- Molecular Weight
- ~40kDa (SDS-PAGE)
- UniProt
- P28776
- Pathways
- Activated T Cell Proliferation
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