FIL1d Protein
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- Target See all FIL1d Proteins
- FIL1d (Interleukin 1 delta (FIL1d))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Purpose
- IL-36Ra (aa 3-156) (mouse) (rec.) (untagged)
- Cross-Reactivity
- Mouse
- Characteristics
- Mouse IL36Ra (aa Val3-Asp156) is untagged.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.05EU/μg purified protein (LAL test).
- Biological Activity Comment
- Inhibits the IL-6 induced expression of IL-36alpha (IL-1F6), IL-36beta (IL-1F8), and IL-36gamma (IL-1F9) in BMDCs. 1μg/ml of IL-36Ra inhibits the activity of 10 ng to 100 ng/ml of the agonists.
- Top Product
- Discover our top product FIL1d Protein
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Interleukin 1 delta (FIL1d) (Active) protein
FIL1d Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 1 delta (FIL1d) (AA 1-155) protein (His tag)FIL1d Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 1 delta (FIL1d) (Transcript Variant 2) (Active) proteinFIL1d Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
Interleukin 1 delta (FIL1d) (AA 1-155), (full length) protein (His-SUMO Tag)FIL1d Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Interleukin 1 delta (FIL1d) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)FIL1d Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Interleukin 1 delta (FIL1d) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)FIL1d Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 100 μL sterile water.
- Concentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- FIL1d (Interleukin 1 delta (FIL1d))
- Alternative Name
- IL-36Ra (FIL1d Products)
- Background
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Interleukin-36 Receptor Antagonist Protein, IL-1F5, Interleukin-1 Family Member 5, IL-1delta, Interleukin-1delta, Interleukin-1 Homolog 3, Interleukin-1-like Protein 1
IL-36Ra/IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to IL-36alpha (IL-1F6), IL-36beta (IL-1F8) and IL-36gamma (IL-1F9). These cytokines bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1beta, IL-6, TNF-alpha and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are target of the IL-36 interleukins leading to a Th1 response. Recently mutations that affect the levels and the activity of IL-36Ra have been found in patients with pustular psoriasis, leading to enhanced production of inflammatory cytokines (IL-8 in particular) by keratinocytes.
- Molecular Weight
- ~17kDa
- UniProt
- Q9QYY1
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