IL-33 Protein (AA 112-270)

Catalog No. ABIN1343983

Product Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 112-270

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Specificity: Binds to human ST2.

Cross-Reactivity: Human

Characteristics: Human IL-33 (aa 112-270) is untagged.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Activates human ST2-dependent NF-kappaB pathway.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

References

Suzukawa, Nagase, Ogahara, Han, Tashimo, Shibui, Koketsu, Nakae, Yamaguchi, Ohta: "Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 9, pp. 5254-60, (2011) (PubMed).

Suzukawa, Iikura, Koketsu, Nagase, Tamura, Komiya, Nakae, Matsushima, Ohta, Yamamoto, Yamaguchi: "An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 9, pp. 5981-9, (2008) (PubMed).

Target Details

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33 (IL33 Products)

Synonyms: IL33 Protein, C9orf26 Protein, DVS27 Protein, IL1F11 Protein, NF-HEV Protein, NFEHEV Protein, RP11-575C20.2 Protein, 9230117N10Rik Protein, Il-33 Protein, Il1f11 Protein, RGD1311155 Protein, interleukin 33 Protein, IL33 Protein, Il33 Protein

Background: Interleukin-33 (IL-33, HF-NEV, IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Molecular Weight: ~18kDa

UniProt: Q2YEJ5

Pathways: Production of Molecular Mediator of Immune Response