Perforin 1 Protein (AA 22-555) (GST tag)

Catalog No. ABIN1316183

Product Details

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Protein Type: Recombinant

Protein Characteristics: AA 22-555

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This Perforin 1 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: PRF1 (Human) Recombinant Protein (P01)

Sequence: PCHTAARSEC KRSHKFVPGA WLAGEGVDVT SLRRSGSFPV DTQRFLRPDG TCTLCENALQ EGTLQRLPLA LTNWRAQGSG CQRHVTRAKV SSTEAVARDA ARSIRNDWKV GLDVTPKPTS NVHVSVAGSH SQAANFAAQK THQDQYSFST DTVECRFYSF HVVHTPPLHP DFKRALGDLP HHFNASTQPA YLRLISNYGT HFIRAVELGG RISALTALRT CELALEGLTD NEVEDCLTVE AQVNIGIHGS ISAEAKACEE KKKKHKMTAS FHQTYRERHS EVVGGHHTSI NDLLFGIQAG PEQYSAWVNS LPGSPGLVDY TLEPLHVLLD SQDPRREALR RALSQYLTDR ARWRDCSRPC PPGRQKSPRD PCQCVCHGSA VTTQDCCPRQ RGLAQLEVTF IQAWGLWGDW FTATDAYVKL FLGGQELRTS TVWDNNNPIW SVRLDFGDVL LATGGPLRLQ VWDQDSGRDD DLLGTCDQAP KSGSHEVRCN LNHGHLKFRY HARCLPHLGG GTCLDYVPQM LLGEPPGNRS GAVW

Characteristics: Human PRF1 full-length ORF ( AAH63043.1, 22 a.a. - 555 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Lu, Lamontagne, Sun, Pinkerton, Block, Lu: "Role of the inflammatory protein serine protease inhibitor Kazal in preventing cytolytic granule granzyme A-mediated apoptosis." in: Immunology, Vol. 134, Issue 4, pp. 398-408, (2011) (PubMed).

Target Details

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1 (PRF1 Products)

Synonyms: FLH2 Protein, HPLH2 Protein, P1 Protein, PFN1 Protein, PFP Protein, PRF1 Protein, Pfn Protein, Pfp Protein, Prf-1 Protein, Cyta Protein, RATCYTA Protein, LOC443187 Protein, perforin Protein, prf1 Protein, cytolysin Protein, perforin-1 Protein, perforin-1-like Protein, perforin 1 Protein, perforin 1 (pore forming protein) Protein, perforin Protein, perforin 1 L homeolog Protein, PRF1 Protein, Prf1 Protein, LOC443187 Protein, prf1 Protein, prf1.L Protein

Background: Full Gene Name: perforin 1 (pore forming protein)
Synonyms: FLH2,HPLH2,MGC65093,P1,PFN1,PFP

Gene ID: 5551

Pathways: Apoptosis, Caspase Cascade in Apoptosis