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Protein A/G Protein (Sepharose)

Origin: Streptococcus, Staphylococcus aureus IP
Catalog No. ABIN1118464
  • Target See all Protein A/G products
    Protein A/G
    Origin
    Streptococcus, Staphylococcus aureus
    Host
    Please inquire
    Purification tag / Conjugate
    This Protein A/G protein is labelled with Sepharose.
    Application
    Immunoprecipitation (IP)
    Specificity
    The product can be used for binding IgG from human, rabbit, goat, mouse and rat. It has strong binding of human, rabbit, cow, sheep, and goat polyclonal antibodies, mouse IgG2a, IgG2b, IgG3, and rat IgG2a. Protein A/G also has moderate affinity for mouse and rat IgG1 and IgG2c. It binds with weak affinity to rat IgG2b. Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies.
    Characteristics
    The conjugate is provided as a suspension with 0.5 ml sepharose per 1 ml of suspension and is blocked with BSA to reduce non-specific immunoglobulin binding.
  • Application Notes
    Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.
    Comment

    Protein A/G resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 µL per immunoprecipitation reaction.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    20% (v/v) Ethanol
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    Protein A/G
    Abstract
    Protein A/G Products
    Background
    Sepharose™ Protein A/G can be used for immunoprecipitation and purification of monoclonal antibodies. Sepharose™ Protein A/G is a suspension of beads conjugated to a fusion protein containing binding domains from both protein A (from Staphylococcus aureaus) and protein G (from Streptococcus sp.). When evenly dispersed approximately 2.0 ml of suspension is equal to 0.5 cc of settled beads of Sepharose™ 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 µm when swollen. Each cc of drained beads will bind approximately 20-30 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to 10. Up to 100 reactions.
    Synonyms: Sepharose Protein A/G, agarose, immunoprecipitation, IP western blot
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