Neuregulin 4 Protein (NRG4)

Catalog No. ABIN1111850

Product Details

Target: Neuregulin 4 (NRG4)

Protein Type: Recombinant

Origin: Human

Host: Please inquire

Application: Biochemical Assay (BCA)

Purification: >96%, as determined by SDS-PAGE and HPLC

Endotoxin Level: Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg)

Biological Activity Comment: The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be <3ng/ml, corresponding to a specific activity of 3x 105 Units/mg.

Application Details

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

References

McElroy, Castle, Bernard, Almohazey, Hunter, Bell, Al Alam, Wang, Ford, Frey: "The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis." in: The American journal of pathology, Vol. 184, Issue 10, pp. 2768-78, (2016) (PubMed).

Ng, Lee, Supko, Khan, Torres, Berwick, Ho, Kirkwood, Siegfried, Stabile: "Pan-erbB inhibition potentiates BRAF inhibitors for melanoma treatment." in: Melanoma research, Vol. 24, Issue 3, pp. 207-18, (2014) (PubMed).

Hayes, Blackburn, Boyle, Russell, Frost, Morgan, Gullick: "Expression of neuregulin 4 splice variants in normal human tissues and prostate cancer and their effects on cell motility." in: Endocrine-related cancer, Vol. 18, Issue 1, pp. 39-49, (2010) (PubMed).

Target Details

Target: Neuregulin 4 (NRG4)

Alternative Name: Neuregulin-4 (NRG4 Products)

Synonyms: HRG4 Protein, AI552600 Protein, neuregulin 4 Protein, NRG4 Protein, Nrg4 Protein, LOC100347220 Protein

Background: Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

Molecular Weight: Recombinant human Neuregulin-4 EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits, and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

NCBI Accession: Q8WWG1

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway