ICT1 Protein (His tag)
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- Target See all ICT1 Proteins
- ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ICT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ICT1 Protein
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- Comment
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Synonyms: Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))
- Alternative Name
- ICT1 (ICT1 Products)
- Synonyms
- ICT1 Protein, DS-1 Protein, DS1 Protein, 1110001A02Rik Protein, 1110002E03Rik Protein, mitochondrial ribosomal protein L58 Protein, imitochondrial ribosomal protein L58 S homeolog Protein, Immature colon carcinoma transcript 1 Protein, MRPL58 Protein, mrpl58.S Protein, PVX_091510 Protein, mrpl58 Protein, Mrpl58 Protein, ict1 Protein
- Background
- Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 22.8 kDa (200aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001536
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