MYL12B Protein (His tag)
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- Target See all MYL12B Proteins
- MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MYL12B protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product MYL12B Protein
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- Comment
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Synonyms: Myosin, light chain 12B regulatory, MLC-B, MRLC2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol, 2 mM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MYL12B (Myosin, Light Chain 12B, Regulatory (MYL12B))
- Alternative Name
- MYL12B (MYL12B Products)
- Synonyms
- Mrlc2 Protein, Mrlcb Protein, Mylc2b Protein, MLC-B Protein, MRLC2 Protein, 1500001M02Rik Protein, C77744 Protein, RLC-B Protein, MYL12B Protein, mlc-b Protein, mrlc2 Protein, mrlcb Protein, myl12b Protein, myosin light chain 12B Protein, myosin, light chain 12B, regulatory Protein, Myosin regulatory light chain 2 Protein, myosin regulatory light chain 2 Protein, myosin regulatory light chain 12B Protein, myosin light chain 12B L homeolog Protein, Myl12b Protein, MYL12B Protein, mlc-2 Protein, MLC-2 Protein, LOC102175348 Protein, LOC101105123 Protein, LOC100062440 Protein, myl12b.L Protein
- Background
- Myosin, light chain 12B, regulatory, also known as MYL12B, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL12B regulates contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases Actin-activated myosin ATPase activity, thereby regulating contractile activity. Recombinant human MYL12B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 22.3 kDa (196aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001138417
- Pathways
- Feeding Behaviour
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