S100A5 Protein (His tag)
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- Target See all S100A5 Proteins
- S100A5 (S100 Calcium Binding Protein A5 (S100A5))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This S100A5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product S100A5 Protein
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- Comment
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Synonyms: S100 calcium binding protein A5, S100D9
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer, pH 8.0, 40% glycerol, 3mM DTT, 200 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- S100A5 (S100 Calcium Binding Protein A5 (S100A5))
- Alternative Name
- S100A5 (S100A5 Products)
- Synonyms
- S100D Protein, S100D9 Protein, S100A5 Protein, S100 calcium binding protein A5 Protein, S100A5 Protein, S100a5 Protein
- Background
- S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. It is expressed in very restricted regions of the adult brain. Recombinant mouse S100A5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 13.4kDa (117aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_035442
- Pathways
- S100 Proteins
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