MDH protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- Target
- MDH
- Protein Type
- Recombinant
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Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
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MDH (AA 1-312), (full length) protein (His tag)
Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
MDH (AA 2-312) protein (His tag)Origin: Bacillus subtilis Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Comment
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Synonyms: malate dehydrogenase, ECK3225, JW3205
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MDH
- Synonyms
- MDH Protein, MMDH Protein, malate dehydrogenase 2 Protein, MDH2 Protein
- Background
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 34.9kDa (336aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_417703
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