Protein phosphatase 1 (AA 1-147), (Regulatory Subunit 14A) protein (His tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- Protein phosphatase 1
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-147, Regulatory Subunit 14A
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A/PPP1R14A (C-6His)
- Sequence
- MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHPLEH HHHHH
- Characteristics
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A/PPP1R14A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro147) of Human PPP1R14A.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Protein phosphatase 1 (Regulatory Subunit 15A) protein
Origin: Rat Host: Escherichia coli (E. coli) Recombinant SDS, WB, ELISA, IP
Protein phosphatase 1 (Regulatory Subunit 15A) proteinOrigin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, WB, ELISA, IP
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 0.2 mM EDTA, 1 mM DTT, 10 % Glycerol, pH 8.0.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- Protein phosphatase 1
- Synonyms
- Ppp1cb Protein, protein phosphatase 1 catalytic subunit beta Protein, PPP1CB Protein
- Background
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Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime, PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated, PPP1R14A has over 1000-fold higher inhibitory activity, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition, inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.
Alternative Names: Protein Phosphatase 1 Regulatory Subunit 14A, 17 kDa PKC-Potentiated Inhibitory Protein of PP1, Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa, CPI-17, PPP1R14A, CPI17, PPP1INL - Molecular Weight
- 17.76 kDa
- UniProt
- Q96A00
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