PDILT Protein (AA 21-584) (His tag)
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- Target See all PDILT Proteins
- PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-584
- Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PDILT protein is labelled with His tag.
- Purpose
- Recombinant Human Protein Disulfide-Isomerase-Like Protein of the Testis/PDILT (C-6His)
- Sequence
- SPEVNAGVSS IHITKPVHIL EERSLLVLTP AGLTQMLNQT RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK VDITIEKELQ QEFGITKAPE LKLFFEGNRS EPISCKGVVE SAALVVWLRR QISQKAFLFN SSEQVAEFVI SRPLVIVGFF QDLEEEVAEL FYDVIKDFPE LTFGVITIGN VIGRFHVTLD SVLVFKKGKI VNRQKLINDS TNKQELNRVI KQHLTDFVIE YNTENKDLIS ELHIMSHMLL FVSKSSESYG IIIQHYKLAS KEFQNKILFI LVDADEPRNG RVFKYFRVTE VDIPSVQILN LSSDARYKMP SDDITYESLK KFGRSFLSKN ATKHQSSEEI PKYWDQGLVK QLVGKNFNVV VFDKEKDVFV MFYAPWSKKC KMLFPLLEEL GRKYQNHSTI IIAKIDVTAN DIQLMYLDRY PFFRLFPSGS QQAVLYKGEH TLKGFSDFLE SHIKTKIEDE DELLSVEQNE VIEEEVLAEE KEVPMMRKGL PEQQSPELEN MTKYVSKLEE PAGKKKTSEE VVVVVAKPKG PPVQKKKPKV KEELVDHHHH HH
- Characteristics
- Recombinant Human Protein Disulfide-Isomerase-Like Protein of the Testis/PDILT produced by transfected human cells is a secreted protein with sequence (Ser21-Leu584) of Human PDILT fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- PDILT (Protein Disulfide Isomerase-Like, Testis Expressed (PDILT))
- Alternative Name
- pdilt (PDILT Products)
- Synonyms
- 1700007B13Rik Protein, RGD1307822 Protein, PDIA7 Protein, protein disulfide isomerase-like, testis expressed Protein, protein disulfide isomerase like, testis expressed Protein, Pdilt Protein, PDILT Protein
- Sub Type
- Fusionprotein
- Background
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Protein Disulfide-Isomerase-Like Protein of the Testis (PDILT) is a protein that belongs to the protein disulfide isomerase family. Human PDILT is synthesized as a 584 amino acid precursor that contains an 20 amino acid signal sequence and a 564 amino acid mature chain. PDILT contains 1 thioredoxin domain lacks the conserved redox-active Cys at position 417 which is replaced by a Ser residue, suggesting that it lacks thioredoxin activity. PDILT is an enzyme in the endoplasmic reticulum in eukaryotes. It is not a disulfide-linked homodimer. The PDILT protein can interacts with ERO1L and CLGN. PDILT probable redox-inactive chaperone involved in spermatogenesis.
Alternative Names: Protein Disulfide-Isomerase-Like Protein of the Testis, PDILT - Molecular Weight
- 65.54 kDa
- UniProt
- Q8N807
- Pathways
- Cell RedoxHomeostasis
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