Pth-Hormone (AA 1-84) (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Func
Active
-
- Target
- Pth-Hormone
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-84
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Purpose
- Recombinant Human Parathyroid Hormone/Parathormone/PTH (1-84)
- Sequence
- SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ
- Characteristics
- Recombinant Human Parathyroid Hormone/PTH produced in E. coli is a single non-glycosylated polypeptide chain containing 84 amino acids with a molecular mass of 9 kDa.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
-
- Comment
-
Biological activity: Recombinant PTH is fully biologically active when compared to standards.Specific Activity of 1.0 x 104 IU/mg as calculated by the UMR106 cell/cAMP method.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 5 % Mannitol, pH 4.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
-
- Target
- Pth-Hormone
- Background
-
Parathyroid hormone is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor as parathyroid hormone and has major effects on development. Like most other protein hormones, parathyroid hormone is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid hormone is secreted as a linear protein of 84 amino acids.
Alternative Names: Parathyroid Hormone, PTH, Parathormone, Parathyrin - Molecular Weight
- 9 kDa
- UniProt
- P01270
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