MICA Protein (AA 24-308) (His tag)
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- Target See all MICA Proteins
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-308
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This MICA protein is labelled with His tag.
- Purpose
- Recombinant Human MHC Class I Polypeptide-Related Sequence A/MICA (C-6His)
- Sequence
- EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETEEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSHWQVDHHH HHH
- Characteristics
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Recombinant Human MHC Class I Polypeptide-Related Sequence A/MICA (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product MICA Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Alternative Name
- MHC Class I Polypeptide-Related Sequence A/MICA (MICA Products)
- Synonyms
- MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein
- Background
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Recombinant Human MHC Class I Polypeptide-Related Sequence A/MICA is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Glu24-Gln308) of Human MICA fused with a polyhistidine tag at the C-terminus.
MHC Class I Polypeptide-Related Sequence A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. Unlike classical MHC class I molecules, MICA does not form a heterodimer with β-2-microglobulin. MICA shares 85 % AA identity with a closely related protein, MICB. MICA acts as a stress-induced self-antigen that is recognized by NK cells, NKT cells, and most of the subtypes of T cells. As a Ligand for the KLRK1/NKG2D receptor, MICA binds to KLRK1 leads to cell lysis. MICA functions as an antigen for γ δ T cells and is frequently expressed in epithelial tumors. MICA antigens are able to elicit the synthesis of alloantibodies in transplant recipients. Studies have shown that anti-MICA antibodies are associated with acute renal allograft rejection and failure. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. - Molecular Weight
- 33.87 kDa
- UniProt
- Q29983
- Pathways
- Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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