NIP7 Protein (AA 1-180) (His tag)
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- Target See all NIP7 Proteins
- NIP7 (Nuclear Import 7 Homolog (NIP7))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-180
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NIP7 protein is labelled with His tag.
- Purpose
- Recombinant Human NIP7/KD93 (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MRPLTEEETR VMFEKIAKYI GENLQLLVDR PDGTYCFRLH NDRVYYVSEK IMKLAANISG DKLVSLGTCF GKFTKTHKFR LHVTALDYLA PYAKYKVWIK PGAEQSFLYG NHVLKSGLGR ITENTSQYQG VVVYSMADIP LGFGVAAKST QDCRKVDPMA IVVFHQADIG EYVRHEETLT
- Characteristics
- Recombinant Human 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog/NIP7 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr180) of Human NIP7 fused with a 6His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product NIP7 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0 .
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- NIP7 (Nuclear Import 7 Homolog (NIP7))
- Alternative Name
- KD93 (NIP7 Products)
- Synonyms
- HSPC031 Protein, KD93 Protein, CGI-37 Protein, Nip7p Protein, pEachy Protein, zgc:110384 Protein, nip7 Protein, Nip7 Protein, ACYPI003208 Protein, NIP7 Protein, DKFZp468F182 Protein, 1110017C15Rik Protein, 6330509M23Rik Protein, AA408773 Protein, AA410017 Protein, PEachy Protein, NIP7, nucleolar pre-rRNA processing protein Protein, NIP7, nucleolar pre-rRNA processing protein L homeolog Protein, 60S ribosome subunit biogenesis protein NIP7 homolog Protein, NIP7 Protein, Nip7 Protein, nip7.L Protein, nip7 Protein
- Sub Type
- Fusionprotein
- Background
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60S Ribosome Subunit Biogenesis Protein NIP7 Homolog (NIP7) belongs to the NIP7 family. NIP7 contains one PUA domain, it is essential for the process of proper 27S pre-rRNA and 60S ribosome subunit assembly. NIP7 is a monomer form and interacts with NOL8 and SBDS, and may bind to RNA. In addition, NIP7 is one of the many trans-acting factors required for eukaryotic ribosome biogenesis, which interacts with nascent pre-ribosomal particles and dissociates as they complete maturation and are exported to the cytoplasm.
Alternative Names: 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog, KD93, NIP7 - Molecular Weight
- 22.6 kDa
- UniProt
- Q9Y221
- Pathways
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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