IL17F Protein (Homodimer)

Catalog No. ABIN1096909

Product Details

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Homodimer

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purpose: Recombinant Human Interleukin-17F/IL-17F

Sequence: MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ

Characteristics: Recombinant Human Interleukin-17F/IL-17F produced in E. coli is a disulfide-linked homodimer of 30.1 kDa, consisting of two non-glycosylated polypeptide chains with 134 amino acids each.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: ED50 is approximately 10 ng/ml as determined by the dose-dependent induction of IL-6 in NIH-3T3 cells.Specific Activity of 1.0 x 105 IU/mg

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 500 mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: il-17f (IL17F Products)

Synonyms: CANDF6 Protein, IL-17F Protein, ML-1 Protein, ML1 Protein, C87042 Protein, IL17F Protein, interleukin 17F Protein, IL17F Protein, Il17f Protein

Background: Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. Today, IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.
Alternative Names: Interleukin-17F, IL-17F, Cytokine ML-1, Interleukin-24, IL-24, IL17F, IL24

Molecular Weight: 30.1 kDa

UniProt: Q96PD4

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity