IFN-alpha/beta R1 (AA 28-436) protein (His tag)
Origin: Human
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- IFN-alpha/beta R1
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-436
- Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Interferon α/β Receptor 1/IFNAR1 (C-6His)
- Sequence
- KNLKSPQKVE VDIIDDNFIL RWNRSDESVG NVTFSFDYQK TGMDNWIKLS GCQNITSTKC NFSSLKLNVY EEIKLRIRAE KENTSSWYEV DSFTPFRKAQ IGPPEVHLEA EDKAIVIHIS PGTKDSVMWA LDGLSFTYSL VIWKNSSGVE ERIENIYSRH KIYKLSPETT YCLKVKAALL TSWKIGVYSP VHCIKTTVEN ELPPPENIEV SVQNQNYVLK WDYTYANMTF QVQWLHAFLK RNPGNHLYKW KQIPDCENVK TTQCVFPQNV FQKGIYLLRV QASDGNNTSF WSEEIKFDTE IQAFLLPPVF NIRSLSDSFH IYIGAPKQSG NTPVIQDYPL IYEIIFWENT SNAERKIIEK KTDVTVPNLK PLTVYCVKAR AHTMDEKLNK SSVFSDAVCE KTKPGNTSKL DHHHHHH
- Characteristics
- Recombinant Human Interferon-alpha/beta Receptor 1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Lys28-Lys436) of Human IFNAR1 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- IFN-alpha/beta R1
- Sub Type
- Fusionprotein
- Background
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The Interferon-alpha/beta Receptor 1 (IFN-alpha/beta R1) is a receptor which binds Type I Interferons including Interferon-alpha and -beta. It is a cell surface receptor and heteromeric receptor composed of one chain with two subunits referred to as IFNAR1 and IFNAR2. IFN-alpha/beta R1, in association with IFN-alpha/beta R2, is required for propagating antiviral signal transduction triggered by IFN-alpha and IFN-beta. IFN-alpha/beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha/beta R2. Ligands associate with IFN-alpha/beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha/beta R1. IFN-alpha/beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation. Human IFN-alpha/beta R1 contains a nuclear localization signal in its extracellular domain that is required for receptor translocation to the nucleus following interaction with ligand. Interferon stimulation results in an immunologic response that is especially associated with viruses.
Alternative Names: Interferon Alpha/Beta Receptor 1, IFN-R-1, IFN-Alpha/Beta Receptor 1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, CRF2-1, Type I Interferon Receptor 1, IFNAR1, IFNAR - Molecular Weight
- 48.18 kDa
- UniProt
- P17181
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