FEN1 Protein (AA 1-380)
FEN1
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target See all FEN1 Proteins
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-380
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human Flap Endonuclease 1/FEN-1
- Sequence
- MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
- Characteristics
- Recombinant Human Flap Endonuclease 1/FEN-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys380) of Human FEN-1.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product FEN1 Protein
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Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) protein (GST tag)
FEN1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Flap Structure-Specific Endonuclease 1 (FEN1) protein (Myc-DYKDDDDK Tag)FEN1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50 mM NaCl, 1 mM DDT, 10 % Glycerol, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
- Alternative Name
- flap-endonuclease-1 (FEN1 Products)
- Background
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Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Alternative Names: Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2 - Molecular Weight
- 42.59 kDa
- UniProt
- P39748
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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