FCGR2A Protein (AA 34-219) (His tag)

Catalog No. ABIN1096627

Product Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Protein Type: Recombinant

Protein Characteristics: AA 34-219

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This FCGR2A protein is labelled with His tag.

Purpose: Recombinant Human Fc γ RIIa/FCGR2A/CD32a (C-6His)

Sequence: APPKAVLKLE PPWINVLQED SVTLTCQGAR SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIMLRCHSW KDKPLVKVTF FQNGKSQKFS RLDPTFSIPQ ANHSHSGDYH CTGNIGYTLF SSKPVTITVQ VPSMGSSSPM GIIVDHHHHH H

Characteristics: Recombinant Human FcgammaRIIA/FCGR2A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gln34-Val219) of Human FCGR2A fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: cd32a (FCGR2A Products)

Synonyms: CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein

Sub Type: Fusionprotein

Background: Receptors for the Fc region of IgG (FcgammaR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcgammaRs are divided into three classes designated FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcgammaRI binds IgG with high affinity and functions during early immune responses, whereas FcgammaRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcgammaRII (A, B, and C) and one for mouse (FcgammaRIIB), encoding type I transmembrane proteins with ITAM motifs (FcgammaRII A and C) or ITIM motifs (FcgammaRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a), FcgammaRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRgamma, CD32a (FcgammaRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcgamma RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.ReferencesKustiawan I, et al. Preventing adsorption of immunoglobulin G to solid surfaces using poloxamer 407 eliminates artifactual stimulation of neutrophils.PMID: 23545494 http://www.ncbi.nlm.nih.gov/pubmed/23545494
Alternative Names: Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, IgG Fc receptor II-a, CDw32, Fc-Gamma RII-a, Fc-Gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2

Molecular Weight: 21.61kDa

UniProt: P12318