CRISP3 Protein (AA 21-245) (His tag)
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- Target See all CRISP3 Proteins
- CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-245
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CRISP3 protein is labelled with His tag.
- Purpose
- Recombinant Human Cysteine-Rich Secretory Protein 3/CRISP-3/SGP28 (C-6His)
- Sequence
- NEDKDPAFTA LLTTQTQVQR EIVNKHNELR RAVSPPARNM LKMEWNKEAA ANAQKWANQC NYRHSNPKDR MTSLKCGENL YMSSASSSWS QAIQSWFDEY NDFDFGVGPK TPNAVVGHYT QVVWYSSYLV GCGNAYCPNQ KVLKYYYVCQ YCPAGNWANR LYVPYEQGAP CASCPDNCDD GLCTNGCKYE DLYSNCKSLK LTLTCKHQLV RDSCKASCNC SNSIYVDHHH HHH
- Characteristics
- Recombinant Human Cysteine-Rich Secretory Protein 3/CRISP-3 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (N21-Y245) of Human CRISP-3 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
- Alternative Name
- crisp-3 (CRISP3 Products)
- Synonyms
- aln-a Protein, crisp3 Protein, MGC76177 Protein, CRISP3 Protein, Aeg2 Protein, CRISP-3 Protein, CRS3 Protein, SGP28 Protein, dJ442L6.3 Protein, AEG2 Protein, HSP-3 Protein, cysteine-rich secretory protein 3 Protein, cysteine-rich secretory protein 3 S homeolog Protein, cysteine rich secretory protein 3 Protein, cysteine-rich secretory protein 2 Protein, CRISP3 Protein, crisp3.S Protein, crisp3 Protein, CpipJ_CPIJ001507 Protein, Crisp3 Protein, LOC481834 Protein
- Sub Type
- Fusionprotein
- Background
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Cysteine-rich secretory protein 3 (CRISP-3) is a secreted protein,containing 1 SCP domain and 1 ShKT domain.It is belongs to the CRISP family. CRISP-3 is a glycoprotein that belongs to the family of cysteine-rich secretory proteins (CRISPs) which was originally discovered in human neutrophilic granulocytes. CRISP-3 is also widely distibuted in exocrine glands (salivary glands, pancreas and prostate), eosinophilic granulocytes and to a lower level in epididymis, ovary, thymus and colon. The presence of CRISP-3 in neutrophils, eosinophils and in exocrine secretions indicates a role in innate host defense. The antibody has been raised against recombinant C-terminally truncated form of CRISP-3 and recognizes both the N-glycosylated and non-glycosylated form of the mature protein.
Alternative Names: Cysteine-Rich Secretory Protein 3, CRISP-3, Specific Granule Protein of 28 kDa, SGP28, CRISP3 - Molecular Weight
- 26.54 kDa
- UniProt
- P54108
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