CD47 Protein (CD47) (AA 19-139) (His tag)
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- Target See all CD47 Proteins
- CD47
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-139
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CD47 protein is labelled with His tag.
- Purpose
- Recombinant Human CD47/IAP/OA3 (C-6His)
- Sequence
- QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PVDHHHHHH
- Characteristics
- Recombinant Human CD47 produced by transfected human cells is a secreted protein with sequence (Gln19-Pro139) of Human CD47 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CD47 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-Citrate, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- CD47
- Alternative Name
- CD47 (CD47 Products)
- Synonyms
- IAP Protein, MER6 Protein, OA3 Protein, 9130415E20Rik Protein, AA407862 Protein, AI848868 Protein, AW108519 Protein, B430305P08Rik Protein, Itgp Protein, CD47/IAP Protein, alkaline phosphatase, intestinal Protein, CD47 molecule Protein, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Protein, Cd47 molecule Protein, ALPI Protein, CD47 Protein, Cd47 Protein
- Sub Type
- Fusionprotein
- Background
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CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Alternative Names: Leukocyte Surface Antigen CD47, Antigenic Surface Determinant Protein OA3, Integrin-Associated Protein, IAP, Protein MER6, CD47, MER6 - Molecular Weight
- 14.76 kDa
- UniProt
- Q08722
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