Prss28 Protein (AA 27-274) (His tag)
PRSS28
Origin: Mouse
Host: Baculovirus infected Insect Cells
Recombinant
> 90 % by SDS - PAGE
SDS
-
- Target
- Prss28 (PRSS28) (Protease, Serine, 28 (PRSS28))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-274
- Origin
- Mouse
- Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Prss28 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPMPTPSAS SPQPKGFRRA VSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLF SLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVR SAREDKVPWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTK EEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYRE DSIPQLEDVS HFLKERTGFQ LRPVAGLLSA RDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL GASDEEIEKL STVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSES FSDAKDKLRN YASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI SHHHHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- Prss28 (PRSS28) (Protease, Serine, 28 (PRSS28))
- Alternative Name
- Prss28
- Synonyms
- Isp1 Protein, mIsp-1 Protein, protease, serine 28 Protein, Prss28 Protein
- Background
- Th, also known as tyrosine 3-monooxygenase, is a rate-limiting enzyme in catecholamine synthesis. It uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. It regulates dopamine (DA) neurotransmission at the biosynthesis and reuptake steps. It plays an important role in the physiology of adrenergic neurons. It effects overexpression in lymphocytes on the differentiation and function of T helper cells. Recombinant mouse Th, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 57.0kDa (507aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_033403
- UniProt
- P24529
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