Proline Transporter antibody (C-Term)
Reactivity: Rat, Human
ELISA, WB, IP
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Proline Transporter
- Binding Specificity
- C-Term
- Reactivity
- Rat, Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- The 24-aa rat peptide used in this study shows 100 % homology with the human transporter. Antibodies made to this epitope have been shown to cross-react with human Proline transporter. Actual cross-reactivity of antibodies in all species is not established.
- Purification
- Immunoaffinity purified
- Immunogen
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A 24 AA peptide, near the C-terminal, cytoplasmic domain was synthesized, coupled to KLH.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
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- Application Notes
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Approved: ELISA (1:100000), IP, WB (1:1000 - 1:5000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation. Western Blot: 1:1000-1:5000 for neat serum and 1-10 μg/mL for affinity pure using ECL. An antibody raised against this epitope has been shown to recognize a ~68kD band in rat and human brain. Deglycosylation reduces the MW to approx. 53kD. ELISA (1:100K using 50-100 ng PROL11 control peptide/well. Immunoprecipitations We recommend the use of affinity pure antibodies at 2-10 μg affinity pure antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % BSA
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Proline Transporter
- Synonyms
- Prot antibody, solute carrier family 6 member 7 antibody, Slc6a7 antibody
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