PRDX6-PS2 antibody (C-Term)
PRDX6-PS2
Reactivity: Arabidopsis thaliana
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
(2 references)-
- Target
- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
- Binding Specificity
- C-Term
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 25 / 20 kDa
- Purification
- serum
- Immunogen
- thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of AtGPX7 accession At4g31870 has been used for antibody production
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- Application Notes
- 1: 5000 with alkaline phosphatase system (AP) (WB), 1:1, 1:2, 1:4, 1:8, 1: 16 (ELISA)
- Comment
-
This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 μL of sterile water.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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-
: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
: "Decarbonylated cyclophilin A Cpr1 protein protects Saccharomyces cerevisiae KNU5377Y when exposed to stress induced by menadione." in: Cell stress & chaperones, (2010) (PubMed).
-
: "Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods." in: Journal of experimental botany, Vol. 64, Issue 12, pp. 3843-54, (2013) (PubMed).
-
- Target
- PRDX6-PS2 (Peroxiredoxin 6 Pseudogene 2 (PRDX6-PS2))
- Alternative Name
- GPX
- Background
-
AGI Code: At4g31870
GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX. - Molecular Weight
- expected: 25 kDa, apparent: 20 kDa
- UniProt
- Q9SZ54
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