DYKDDDDK Tag Peptide
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- Target See all DYKDDDDK Tag products
- DYKDDDDK Tag
- Peptide Type
- Synthetic
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Source
- Synthetic
- Application
- Immunoprecipitation (IP), SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 000-000-h47
- Supplier
- Rockland
- Purpose
- Triple FLAG® Peptide
- Characteristics
- synthetic FLAG® triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide, control peptide, blocking peptide
- Purity
- Greater than 90% specific peptide by HPLC and MS.
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DYKDDDDK Tag peptide
Host: Synthetic IP, SDS, WB Without preservative
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- Application Notes
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Application Note: The Triple FLAG® Peptide has been tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 μg per 1.0 μL of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for recommendations for use.
Western Blot Dilution: YES as blocking peptide in PCA
Immunoprecipitation Dilution: YES - see PROTOCOL
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Reconstitution Volume: 1.0 mL
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: None
Stabilizer: None
Preservative: None - Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
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- Target
- DYKDDDDK Tag
- Target Type
- Tag
- Background
- Background: The Triple FLAG® Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG® sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK). The triple FLAG peptide is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free triple FLAG® Peptide.
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