PCDHG antibody (C-Term, Cytoplasmic Domain, pan)
PCDHG
Reactivity: Mouse, Rat
ICC, IF, WB
Host: Mouse
Monoclonal
S159-5
unconjugated
-
- Target
- PCDHG (Protocadherin gamma Cluster (PCDHG))
- Binding Specificity
- AA 808-931, C-Term, Cytoplasmic Domain, pan
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
- Specificity
- This antibody detects a ~100 kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins. Species: Mouse and Rat.
- Purification
- Protein G Chromatography
- Immunogen
- Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A-subfamily: amino acids ~807-930, B-subfamily: amino acids ~789-912 and C subfamily).
- Clone
- S159-5
- Isotype
- IgG1
-
- Application Notes
- Western blot: 1/1000, 1 μg/mL was sufficient for detection of PCDHG (pan) in 20 μg of Rat brain lysate by colorimetric immunoblot analysis using HRP conjugated secondary antibody. Immunocytochemistry .
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
-
- Target
- PCDHG (Protocadherin gamma Cluster (PCDHG))
- Alternative Name
- pcdhg
- Background
- The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
- Gene ID
- 93709
- UniProt
- Q91XZ0
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