Human BMP-7 EZ Set™ ELISA Kit (DIY Antibody Pairs)
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- Target See all BMP7 products
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- Binding Specificity
- AA 293-431
- Reactivity
- Human
- Detection Method
- Colorimetric
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Host
- Rabbit
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-7 in Human samples.
- Brand
- EZ-Set™
- Specificity
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Expression system for standard: NSO
Immunogen sequence: S293-H431 - Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
- Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
- Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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- Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers - Restrictions
- For Research Use only
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- Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Expiry Date
- 12 months
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- Target
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- Alternative Name
- BMP7 (BMP7 Products)
- Synonyms
- bmp-7 Matched Antibody Pair, bmp7 Matched Antibody Pair, op-1 Matched Antibody Pair, BMP7 Matched Antibody Pair, zgc:153025 Matched Antibody Pair, OP-1 Matched Antibody Pair, OP1 Matched Antibody Pair, BMP-7 Matched Antibody Pair, fb83f09 Matched Antibody Pair, wu:fb83f09 Matched Antibody Pair, bone morphogenetic protein 7 Matched Antibody Pair, bone morphogenetic protein 7, gene 1 L homeolog Matched Antibody Pair, bone morphogenetic protein 7b Matched Antibody Pair, bone morphogenetic protein 7, gene 2 L homeolog Matched Antibody Pair, bone morphogenetic protein 7a Matched Antibody Pair, BMP7 Matched Antibody Pair, bmp7.1.L Matched Antibody Pair, LOC100136282 Matched Antibody Pair, bmp7b Matched Antibody Pair, Bmp7 Matched Antibody Pair, LOC100008826 Matched Antibody Pair, bmp7.2.L Matched Antibody Pair, bmp7a Matched Antibody Pair
- Background
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Background: Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
Synonyms: Bone morphogenetic protein 7 (Osteogenic protein 1), isoform CRA_b ,cDNA FLJ78019, highly similar to Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1), mRNA ,cDNA, FLJ92758, Homo sapiens bone morphogenetic protein 7 (osteogenic protein 1)(BMP7), mRNA ,BMP7 ,hCG_40100 ,
Full Gene Name: bone morphogenetic protein 7
- UniProt
- A8K571
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