p73 gamma Protein (GST tag)
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- Target
- p73 gamma (TP73 gamma) (Tumor Protein p73 gamma (TP73 gamma))
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This p73 gamma protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-s72s
- Supplier
- Rockland
- Purpose
- p73 gamma recombinant protein-GST fusion protein
- Purification
- Full-length recombinant human p73 gamma was expressed by baculovirus in Sf9 cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- Purity
- >90%
-
- Application Notes
-
Western_Blot_Dilution: User Optimized
Application_Note: p73 gamma Protein is suitable for use in Western Blot. Expect a band approximately ~85 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- p73 gamma Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
-
- Target
- p73 gamma (TP73 gamma) (Tumor Protein p73 gamma (TP73 gamma))
- Alternative Name
- p73 gamma
- Background
-
Synonyms: TP73, TAp73alpha, Tumor protein p73, p53-like transcription factor, p53-related protein
Background: p73 gamma is a member of the p53 family of transcription factors which are involved in cellular responses to stress and development. There are four isoforms of p73 (p73 alpha, p73 beta, p73 delta and p73 gamma) which differed in their abilities to form homodimeric and heterodimeric interactions with each other and with p53 (1). The p73 isoforms also differed in their abilities to activate transcription of the p21 (Waf1) promoter and to inhibit colony formation. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. p73 is a stress-response gene that activates transcription of p53-responsive genes and inhibits cell growth in a p53-like manner by inducing apoptosis (2). p73 gamma Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, and p38 Pathway research.
- UniProt
- O15350-3
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