Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) antibody
Verified
NR1H2/3
Reactivity: Human
ELISA, FACS, IF, WB
Host: Goat
Polyclonal
unconjugated
-
- Target See all Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) products
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- Binding Specificity
- AA 429-442, AA 443-456
- Reactivity
- Human
- Host
- Goat
- Clonality
- Polyclonal
- Application
- ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
- Purpose
- LXR alpha / LXR beta
- Sequence
- CRLQDKKLPP LLSEI
- Specificity
- This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
- Isotype
- IgG
-
- Application Notes
-
Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)
- Alternative Name
- NR1H3, NR1H2
- Synonyms
- LXR-a antibody, LXRA antibody, RLD-1 antibody, nuclear receptor subfamily 1 group H member 3 antibody, NR1H3 antibody
- Background
- NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
- Gene ID
- 10062, 7376
- NCBI Accession
- NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
- Pathways
- Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
-
You are here:
