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Ngp antibody (C-Term)

NGP Reactivity: Mouse, Rabbit FM, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5706813
  • Target
    Ngp (NGP) (Neutrophilic Granule Protein (NGP))
    Binding Specificity
    C-Term
    Reactivity
    Mouse, Rabbit
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Fluorescence Microscopy (FM), Western Blotting (WB)
    Characteristics

    Synonyms: rabbit anti-Ngp antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein

    Background: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.

    Gene Name: Ngp

    Purification
    Anti-Ngp is directed against full-length mouse Ngp. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.
    Immunogen

    Immunogen: Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in IF, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to Ngp by western blotting in the appropriate cell lysate or extract. 

    Western Blot Dilution: 1:1000

    IF Microscopy Dilution: 1:200

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Hong, Qu, Wuest, Huang, Huang, Lin: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).

  • Target
    Ngp (NGP) (Neutrophilic Granule Protein (NGP))
    Alternative Name
    Ngp
    Synonyms
    NGP antibody, bectenecin antibody, neutrophilic granule protein antibody, Ngp antibody
    Gene ID
    18054
    NCBI Accession
    NP_032720
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