Ngp antibody (C-Term)
(1 reference)-
- Target
- Ngp (NGP) (Neutrophilic Granule Protein (NGP))
- Binding Specificity
- C-Term
- Reactivity
- Mouse, Rabbit
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Fluorescence Microscopy (FM), Western Blotting (WB)
- Characteristics
-
Synonyms: rabbit anti-Ngp antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein
Background: This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.
Gene Name: Ngp
- Purification
- Anti-Ngp is directed against full-length mouse Ngp. This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in Ngp based on 100% homology match for the immunogen sequence.
- Immunogen
-
Immunogen: Affinity purified Anti-Ngp antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse Ngp protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
-
- Application Notes
-
Application Note: This affinity purified antibody has been tested for use in IF, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to Ngp by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:200
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.1 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
-
-
: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
-
: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).
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- Target
- Ngp (NGP) (Neutrophilic Granule Protein (NGP))
- Alternative Name
- Ngp
- Synonyms
- NGP antibody, bectenecin antibody, neutrophilic granule protein antibody, Ngp antibody
- Gene ID
- 18054
- NCBI Accession
- NP_032720
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