Neuroligin 1/2/3/4 antibody (AA 1-695)
NLGN1/2/3/4
Reactivity: Mouse, Rat
IP, ELISA, WB
Host: Mouse
Monoclonal
4F9
unconjugated
1 image
(6 references)-
- Target
- Neuroligin 1/2/3/4 (NLGN1/2/3/4)
- Binding Specificity
- AA 1-695
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunoprecipitation (IP), ELISA, Western Blotting (WB)
- Specificity
- Recognizes rat and mouse neuroligins 1-3 and rat neuroligin 4.
- Purification
- purified IgG
- Immunogen
- Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1-695).
- Clone
- 4F9
- Isotype
- IgG2a
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- Application Notes
-
WB: 1 : 1000 (AP staining)
ICC: not recommended
IHC: not recommended - Comment
-
ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).
: "The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis." in: Molecular autism, Vol. 6, pp. 17, (2015) (PubMed).
: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).
: "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20782-7, (2010) (PubMed).
: "A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9667-76, (2006) (PubMed).
: "Control of excitatory and inhibitory synapse formation by neuroligins." in: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, (2005) (PubMed).
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: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." in: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).
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- Target
- Neuroligin 1/2/3/4 (NLGN1/2/3/4)
- Alternative Name
- Neuroligin
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