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- Detection Method
- Colorimetric
- Detection Range
- 10-180 kDa
- Minimum Detection Limit
- 10 kDa
- Application
- SDS-PAGE (SDS), Western Blotting (WB)
- Purpose
- The BlueAQUA Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa.
- Characteristics
- Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).The BlueAQUA Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
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Protein Molecular Weight Marker
14-120 kDa Colorimetric
BLUltra Prestained Protein Ladder6.5-270 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
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- Application Notes
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- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel. - Reagent Preparation
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Quality Control: Under suggested conditions, BlueAQUA Prestained Protein Ladder resolves 11 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Approximately 0.1~0.5 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
- Storage
- -20 °C
- Storage Comment
- Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage, store at -20°C
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