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- Detection Method
- Colorimetric
- Detection Range
- 6.5-270 kDa
- Minimum Detection Limit
- 6.5 kDa
- Application
- SDS-PAGE (SDS), Western Blotting (WB)
- Purpose
- The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa.
- Characteristics
- Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
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Protein Molecular Weight Marker
14-120 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BlueAQUA Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
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- Application Notes
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- 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 2.5 μl per well for general Western transferring. - Reagent Preparation
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Quality Control: Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12 % Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Storage Buffer: 62.5 mM Tris-H3PO4 ( pH 7.5 at 25 °C), 1 mM EDTA, 2 % SDS, 10 mM DTT, 1 mM Sodium azide, 33 % glycerol.
- Storage
- -20 °C
- Storage Comment
- Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C.For long term storage, store at -20°C.
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