BLUeye Prestained Protein Ladder
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- Detection Method
- Colorimetric
- Detection Range
- 10-245 kDa
- Minimum Detection Limit
- 10 kDa
- Application
- SDS-PAGE (SDS), Western Blotting (WB)
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Protein Molecular Weight Marker
14-120 kDa Colorimetric
BLUltra Prestained Protein Ladder6.5-270 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BlueAQUA Prestained Protein Ladder10-180 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
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- Application Notes
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Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane. - Comment
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The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
- Reagent Preparation
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Quality Control:
Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10 % Tris-Glycine Bis-Tris 10 % MES buffer 4~20 % MOPS buffer
- Storage
- RT
- Storage Comment
- Store at 25°C for 6 months, Store at -20°C for 24 months
- Expiry Date
- 24 months
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