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- Detection Method
- Colorimetric
- Detection Range
- 5 kDa - 245 kDa
- Minimum Detection Limit
- 5 kDa
- Application
- SDS-PAGE (SDS), Western Blotting (WB)
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Protein Molecular Weight Marker
14-120 kDa Colorimetric
BLUltra Prestained Protein Ladder6.5-270 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueRAY Prestained Protein Ladder10-180 kDa Colorimetric
BlueAQUA Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
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- Application Notes
- Use 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
- Comment
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The BLUelf Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The BLUelf Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
- Reagent Preparation
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Quality Control:
Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Approximately 0.1~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C. - Expiry Date
- 24 months
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