Heart Membrane Lysate
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- Species of Lysate
- Rhesus Monkey
- Application
- Western Blotting (WB)
- Characteristics
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Monkey (Rhesus) heart tissue membrane protein lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The monkey (Rhesus) heart tissue membrane is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated heart tissue membrane protein is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Source: Monkey (Rhesus) heart tissue
Diagnosis: Normal
Tissue or Cell type: Heart - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Membrane Fraction
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- Application Notes
- Heart membrane lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- Handling Advice
- Repeated freezing and thawing is not recommended.
- Storage
- 4 °C/-80 °C
- Storage Comment
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- Expiry Date
- 6 months
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