Adipose Subcutaneous Diabetic Disease Lysate
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- Species of Lysate
- Human
- Application
- Western Blotting (WB)
- Characteristics
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Human subcutaneous adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70 °C. The human subcutaneous adipose tissue total protein is provided in a buffer including HEPES ( pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the subcutaneous adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The subcutaneous adipose tissue is then Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Source: Human Subcutaneous Adipose tissue
Diagnosis: Diseased
Tissue or Cell type: Adipose - Lysate Type
- Tissue Lysate
- Lysate Fraction
- Whole Tissue Lysate
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- Application Notes
- Adipose Subcutaneous lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.
- Handling Advice
- Repeated freezing and thawing is not recommended.
- Storage
- 4 °C/-80 °C
- Storage Comment
- Store at 2-8 °C for continuous use. For extended storage, freeze working aliquots at -70 °C.
- Expiry Date
- 6 months
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