DYKDDDDK Positive Control Lysate
PC, WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Tag
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- Target See all DYKDDDDK Tag products
- DYKDDDDK Tag
- Protein Species
- Tag
- Species of Lysate
- Human Cells
- Application
- Positive Control (PC), Western Blotting (WB)
- Endotoxin Level
- Low Endotoxin : No
- Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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- Application Notes
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
- Storage
- -80 °C
- Storage Comment
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
- Expiry Date
- Expiration date is three (3) months from date of opening.
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- Target
- DYKDDDDK Tag
- Abstract
- DYKDDDDK Tag Products
- Target Type
- Tag
- Background
- This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
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