LOC373225 antibody
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- Target
- LOC373225 (Similar To CG15792-PD (LOC373225))
- Reactivity
- Human, Mouse, Rat, Turkey
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
- Specificity
- This antibody recognizes myosin II and is highly specific for immunoblotting. Binding assays show strong reactivity with myosin heavy chain, weak reactivity with myosin light chain, and no cross-reactivity with skeletal and smooth muscle myosin. Immunocytochemistry shows uniform staining throughout growth cones in a punctate pattern with occasional concentration in filopodia. Rabbits were immunized with an intact and native myosin II purified from human platelets.
- Purification
- Ammonium sulfate precipitation
- Immunogen
- Myosin II.
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- Application Notes
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Approved: ICC (1:500), IF (1:500), IP, WB (1:5000)
Not recommended for: IHC-P - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Phosphate buffered saline, pH 7.5, 0.015 M sodium phosphate, 0.15 M NaCl, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- LOC373225 (Similar To CG15792-PD (LOC373225))
- Alternative Name
- Myosin II
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