Expected / apparent Molecular Weight of the Antigene: 23 / 23 for Arabidopsis thaliana
Purification
affinity purified
Immunogen
BSA-conjugated synthetic peptide derived from the Lhca4 protein of Arabidopsis thaliana (At1g45474). This sequence is highly conserved in Lhca5 proteins of angiosperms (monocots and dicots) and gymnosperms.
Isotype
IgG
Application Notes
Recommended Dilution: 1 : 1000- 1 : 3000 with standard ECL (WB). This antibody is not suitable for immunohistochemistry.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL of sterile water
Buffer
PBS pH 7.4
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Target
Lhca5
(psi Type V Chlorophyll A/B-Binding Protein (Lhca5))
Background
AGI Code: At1g45474 Lhca5 is a low abundant chlorophyll a/b-binding protein that has been found to associate with Photoystem I in sub-stoechiometric amounts. Unlike the four main Lhca-types (Lhca1-4) Lhca5 seems to be able to associate with PSI or the LHCI at varying positions. The regulation pattern for the expressino of the Lhca5 gene differs from the standard Lhc pattern as expression seems to be positively regulated under light and cold stress conditions.
Molecular Weight
expected: 23 kDa, apparent: 23 for Arabidopsis thaliana